RIA for Serum Holo-Transcobalamin: Method Evaluation in the Clinical Laboratory and Reference Interval
نویسندگان
چکیده
منابع مشابه
RIA for serum holo-transcobalamin: method evaluation in the clinical laboratory and reference interval.
BACKGROUND Decreased serum holo-transcobalamin (holoTC) could be the earliest marker of cobalamin (Cbl) deficiency, but there has been no method suitable for routine use. We evaluated a new commercial holoTC RIA, determined reference values, and assessed holoTC concentrations in relation to other biochemical markers of Cbl deficiency. METHODS The reference population consisted of 303 individu...
متن کاملDirect assay for cobalamin bound to transcobalamin (holo-transcobalamin) in serum.
BACKGROUND Only cobalamin carried by transcobalamin (holo-transcobalamin) is available for cellular uptake and hence is physiologically relevant. However, no reliable or accurate methods for quantifying holo-transcobalamin are available. We report a novel holo-transcobalamin assay based on solid-phase capture of transcobalamin. METHODS A monoclonal antibody specific for human transcobalamin w...
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BACKGROUND Human placenta expresses CD320, a receptor that ensures the uptake of holo-transcobalamin (holoTC). Soluble CD320 (sCD320) is present in the circulation and its concentration increases during pregnancy. AIMS To investigate a possible association of sCD320, holoTC and total transcobalamin (TC) with the risk of subsequent preeclampsia using serum samples from asymptomatic first trime...
متن کاملResampling approach for determination of the method for reference interval calculation in clinical laboratory practice.
Reference intervals (RI) play a key role in clinical interpretation of laboratory test results. Numerous articles are devoted to analyzing and discussing various methods of RI determination. The two most widely used approaches are the parametric method, which assumes data normality, and a nonparametric, rank-based procedure. The decision about which method to use is usually made arbitrarily. Th...
متن کاملBiological Variation of Holo-Transcobalamin in Elderly
Individuals, Andrew McCaddon, Peter Hudson, Cherie McCracken, Richard Ellis, and Anne McCaddon ( University of Wales College of Medicine, Division of General Practice, Wrexham LL13 7YP, UK; 2 Department of Pathology, Wrexham Maelor Hospital, Wrexham LL13 7TD, UK; 3 University Department of Psychiatry, Royal Liverpool University Hospital, Liverpool L69 3GA, UK; 4 University Hospital of Wales, Ca...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2003
ISSN: 0009-9147,1530-8561
DOI: 10.1373/49.3.455